Changes to gut amino acid transporters and microbiome associated with increased E/I ratio in Chd8 +/- mouse model of ASD-like behavior
Posted on: Tuesday, May 3rd, 2022 | ID: #1119

Fig. 4. B. uniformis improves the ASD-like behaviors and restores E/I imbalance in the Chd8…

Fig. 4. B. uniformis improves the ASD-like behaviors and restores E/I imbalance in the Chd8+/− mice.

a Schematic diagram for co-housing experiments. b qPCR analysis of B. uniformis in the feces from 8-week-old mice. n = 26, 16, and 8 mice, respectively. c Percentages of time spent in the light box in the light/dark box test. n = 27, 15, and 8 mice, respectively. d Percentages of the interaction time (left) and the preference index (right) in the social novelty preference session of the three-chamber social interaction test. n = 26, 18, and 8 mice, respectively. e Schematic diagram for B. uniformis gavage. f Percentages of the interaction time (left) and the preference index (right) in the social novelty preference session of the three-chamber social interaction test. n = 12, 11, and 11 mice, respectively. g Representative traces (left) and percentages of time in the center (right) in the open-field test. n = 10, 14, and 11 mice, respectively. h Percentages of time spent in the light box in the light/dark box test. n = 15, 8, and 9 mice, respectively. i, j Representative traces (top), cumulative distribution plots (bottom) and bar graphs (bottom) showing the amplitude and frequency of the mEPSCs (e) and the mIPSCs (f) of pyramidal cells in neocortical layer V. n = 4, 4, and 5 mice, respectively. Four to six data points per mouse. For mEPSCs, n = 17, 16, and 20 data points, respectively; for mIPSCs, n = 18, 22, and 25 data points, respectively. k Levels of L-glutamate (μg/g), glutamate/GABA (Glu/GABA), and L-glutamine (μg/g) in the cerebrum detected by targeted metabolomics assays. n = 16, 9, and 9 mice, respectively. Quantitative data are shown as the mean ± SEM. Statistical analysis was determined by one-way ANOVA with two-tailed Tukey’s multiple comparison test (b, c, d (right panel), f (right panel), g, h, i, j, k) and two-way ANOVA with two-tailed Turkey’s test for multiple comparisons (d (left panel) and f (left panel)). Significance was indicated by P value.

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